Manipulating the Mouse Embryo                             
by Andras Nagy, Marina Gertsenstein, Kristina Vintersten, and Richard Behringer
2003 • 764 pp., illus., paperback, appendices, index

Description
Regarded as the "Bible" of mammalian embryo manipulation techniques since the 1986 publication of the first edition of Manipulating the Mouse Embryo: A Laboratory Manual, the third edition of this essential laboratory resource has now been completely reorganized, rewritten, and updated by a new cast of authors. The result is a compilation of new, cutting–edge protocols that include embryonic stem cell production and genetic manipulation, mouse chimeras, mouse cloning, assisted reproduction strategies (including intracytoplasmic sperm injection and in vitro fertilization), whole embryo culture systems, electroporation, embryo and gamete cryopreservation and rederivation, and gene expression, as well as more extensive background information on the use of these techniques. The "gold standard" techniques for applying recombinant DNA technology to investigations of mammalian embryonic development included in the first two editions of this book are also updated and recast, as is the summary of the current state of understanding of mouse development at the molecular level. This book is the premier authoritative and comprehensive source of technical and theoretical guidance for mouse developmental biologists and geneticists and is an essential resource for newcomers to these fields.

Key features of the third edition

	New authors are leading authorities in mouse development and genetics, as were authors of prior editions.
	All new illustration and new design: All illustrations have been redrawn; extensive use of new illustrations and photographs of techniques and results; a new, contemporary design presents techniques more clearly.
	New explanatory text: More background information for techniques is presented as well as more extensive troubleshooting information.
	New, functional organization and techniques.

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Live Cell Imaging: A Laboratory Manual
Edited By Robert D. Goldman, Northwestern University Medical School, Chicago; David L. Spector
648 pp., illus., appendices, index, DVD
Paperback

Description
Recent advances in imaging technology reveal, in real time and great detail, critical changes in living cells and organisms. This manual is a compendium of emerging techniques, organized into two parts: specific methods such as fluorescent labeling, and delivery and detection of labeled molecules in cells; and experimental approaches ranging from the detection of single molecules to the study of dynamic processes in organelles, organs, and whole animals. Although presented primarily as a laboratory manual, the book includes introductory and background material and could be used as a textbook in advanced courses. It also includes a DVD containing movies of living cells in action, created by investigators using the imaging techniques discussed in the book.

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Mouse Phenotyping: A Handbook of Mutation Analysis
By Virginia E. Papaioannou, Columbia University College of Physicians and Surgeons; Richard R. Behringer, University of Texas MD Anderson Cancer Center
250 pp. (approx.), illus., appendices, index
Concealed wire binding

Description
The generation of mutant mice raises many questions about the best means of phenotypic analysis, breeding, and maintenance. The answers are now available from two experts with a wealth of detailed knowledge never previously assembled in one volume. Informal and highly practical, this handbook provides step–by–step methods for troubleshooting experiments, from the basics of gene targeting through the analysis of postnatal effects.

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